Agricultural Reviews
  • Year: 2024
  • Volume: 45
  • Issue: 4

Impact of RNA Enzymes and RNA Protein Complexes in Unleashing RNA Interference for Manyfold Crop Improvements: A Review

  • Author:
  • Nezif Abajebal1, John Barnabas1,*, Tsegaye Abebe1
  • Total Page Count: 18
  • Page Number: 561 to 578

1Department of Plant Science, College of Agriculture and Natural Resource, Gambella University, Gambella, P.O. Box 126, Ethiopia

*Corresponding Author: John Barnabas, Department of Plant Science, College of Agriculture and Natural Resource, Gambella University, Gambella, P.O. Box 126, Ethiopia, Email: johnbarnabas@gmail.com

Online Published on 03 June, 2025.

Abstract

RNA interference, which enables more accurate downregulation of gene expression without interfering with the expression of various genes, is a beneficial instrument for gene silencing and a potential gene regulatory strategy in functional genomics that has a big impact on crop progress. Small interfering RNA molecules swift up the RNAi gene silencing machinery, effectually suppressing a target gene. Subsequently transgene proteins not present from genetically modified RNAi plants, it has a sum of rewards over orthodox transgenic methods. Dicer, Drosha which are RNA enzymes, along with protein complexes, RISC and Argonaute, institute at the epicenter of this technology. These explicit RNA binding proteins and ribonucleases regulate the synthesis and production of tiny regulating RNAs. Several latent usages for this ground-breaking process in agriculture have been identified for improving crops, such as the construction of disease-resistant, biotic or abiotic, high-yielding, stress-tolerant and exclusive assortments; enhancement of nutritional quality; removal of allergens and toxins and postponing the ripening of fruits and vegetables for extended storage periods. This ground-breaking procedure has the latent to be further utilized to pave the way for the creation of environmentally friendly biotech methods for crop protection and enhancement. Improving research and development activities in this auspicious field is imperative to ensure the efficient appropriate and safe application of these instruments as enduring remedies for agricultural enhancement.

Keywords

Argonaute, Dicer, Drosha RISC, miRNA, RNAi, Silencing path way, siRNA