Department of Genetics, CCS Haryana Agricultural University, Hisar - 125 004, India
Embryos excised from immature grains of two Kamal bunt resistant (HD29 arid WH 283) and three susceptible(HD2329, HD2009 and WH147) genotypes were cultured on Murashige and Skoog (1962) (MS) and Gressh of and Doy (1972) (GD) media supplemented with various growth regulators. Callus was induced within 4.33 to 6.33 days in embryos of all the five genotypes on all the media. Callusing response i.e per cent embryos showing callus induction and regeneration response (% calli showing regeneration) depended upon genotype, media and genotype x media interactions. On an average over media cv WH147 showed maximum (88.92%) callusing response. GD medium supplemented with 2 mg/1 of 2,4-D was the best for callus induction. Maximum regeneration frequency was observed in cv HD 2009. Among different media tried, MS medium supplemented with 0.5 mg/1 of 2,4-D was the test for regeneration in these genotypes.
