In vitro micropropagation studies in sugarcane

The MS (Murashige and Skoog, 1962) medium supplemented with different levels of BAP (6, Benzyl ammo purine) (0.1, 0.2, 1.0, 2.0, 3.0 mg/l) and IBA (Indol-3-butyric acid) (0.1, 0.5, 1.0, 2.0 mg/l) with NAA (Napthaline acetic acid) (0.2, 0.3, 0.5 mg/l) were prepared separately and used for inoculation and subculturing as and when required. The establishment of shoot apex culture was observed on me media containing MS + 0.2 mg/l BAP + 0.2 mg/l NAA in the variety CoM-88121 (70%) and Co-86032 (60%). The maximum number of shoots (9.8 shoots in Co-86032 and 8.1 shoots in CoM-88121) were observed in me medium MS + 1.0 mg/l BAP + 0.5 mg/l NAA. The maximum number of roots were observed in the culture medium ½ MS + 1 mg/l IBA + 0.3 mg/l NAA. For hardening of the plantlets the relative humidity of 70–90% and 28–32°C temperature was maintained for initial 8 to 12 days and within such period the new roots were developed to these plantlets. The 60 days old spellings were planted in the field.