Department of Agricultural Biotechnology, College of Agriculture, Orissa University of Agriculture & Technology, Bhubaneswar-751 003, India
*Corresponding author's e-mail: grrout@rediffmail.com
Online published on 4 October, 2013.
An efficient and rapid regeneration protocol was developed using shoot apices from germinating seedlings of two varieties of indica rice i.e. Ghanteshwari and Siddhanta. Shoot apical meristems from 7-d-old seedlings produced axillary and adventitious shoots on MS media containing 3.0 mg/l 6-benzyladenine (BA). Both the genotypes responded positively on shoot multiplication depending upon media composition. The maximum rate of shoot multiplication was 90%, and the average number of multiple shoots per culture was 12.5 in case of var. Ghanteswari and 10.6 in case of Siddhanta within 4 weeks of culture on 3.0 mg/l BA in combination with 50 mg/l adenine sulphate (Ads). Actively multiplying shoot clumps were recovered with high frequency within 4 weeks of cultures. These excised shoots were rooted on MS medium supplemented with 0.5 mg/l NAA and 3% sucrose. Plantlets were success-fully established in soil. This efficient protocol may be utilized for the production of elite clones and also genetic improvement program
Growth regulator, Micropropagation, Multiple shoots, Rice, Rooting
