1Department of Biotechnology, S G R R P G College, Dehradun, U.K.
2Department of Microbiology, S G R R P G College, Dehradun, U.K.
3Department of Environmental Biology, A.P.S. University, Rewa, M.P.
4Department of Botany, Govt. P.G. Science College, Rewa, M.P.
Department of Biotechnology, SGRRPG College, Dehradun-248 001, U.K.
*Corresponding author's e-mail: naveensri17@gmail.com
Online published on 19 December, 2016.
Secondary metabolite contents of W. somnifera varied remarkably between seasons and genotypes under ex vitro condition. In vitro studies provide an optimum culture condition for steady and quality production of bioactive chemicals throughout the year without involvement of environmental stresses. Mass production of micro-shoots and plantlets by exploring the organogenic totipotency of shoot tip explants (ex vitro and in vitro grown) considering two elite genotypes (Poshita and Jawahar 22) of W. somnifera, and assessment of their capability in production and accumulation of bioactive metabolites (total alkaloid and withanolides amount were quantified; withanolide A and withaferin A contents were estimated by High Performance Liquid Chromatography-HPLC).
Bioactive chemicals, Culture condition, Ex vitro, In vitro, Mass production, Micro-shoots, Secondary metabolite, W. somnifera
