1JSA College of Agriculture and Technology, Tittagudi-606 108, Tamil Nadu
2Department of Genetics and Plant Breeding, Faculty of Agriculture, Annamalai University, Annamalai Nagar, Tamilnadu, India
*Corresponding Author: M. Prakash, Department of Genetics and Plant Breeding, Faculty of Agriculture, Annamalai University, Annamalai Nagar, Tamilnadu, India, Email: geeth_praksh@yahoo.co.in
Online published on 15 October, 2019.
The utilization and conservation of traditional rice genotypes have attracted global attention. Optimization of DNA isolation protocol for genetic characterization of plants is a necessary and primary step. Over the recent years, next-generation sequencing and microarray technologies have revolutionized scientific research, with their applications to high-throughput analysis of biological systems. Isolation of high quantities of pure, intact, double-stranded, highly concentrated DNA is prerequisite for successful and reliable large-scale genotyping analysis. Therefore, standardization of DNA isolation is a basic requirement. Here we employed three methods of DNA isolation, namely, Dellaporta, Hi-purA, and modified CTAB techniques for isolation of genomic DNA from 25 indigenous rice genotypes. From the results, it was found that genomic DNA isolated by a modified CTAB method to be the most appropriate for extracting high quality and maximum quantity of DNA suitable for genotyping. Spectrophotometric measurements and gel electrophoresis subsequently assessed the quality and quantity of the differentially extracted DNA.
Isolation, CTAB, UV nano-spectrophotometer, gel electrophoresis, UV gel documentation
