1Laboratory of Veterinary Clinic, Reproduction, Pathology and Nutrition, Faculty of Medicine and Veterinary Medicine, Universitas Nusa Cendana, Kupang, Indonesia
2Laboratory of Animal Diseases and Veterinary Public Health, Faculty of Medicine and Veterinary Medicine, Universitas Nusa Cendana, Kupang, Indonesia
3Faculty of Animal Husbandry, Marine and Fisheries, Universitas Nusa Cendana, Kupang, Indonesia
4Laboratory of Veterinary Anatomy, Physiology, Pharmacology and Biochemistry, Faculty of Medicine and Veterinary Medicine, Universitas Nusa Cendana, Kupang, Indonesia
*Corresponding Author: Cynthia Dewi Gaina, Laboratory of Veterinary Clinic, Reproduction, Pathology and Nutrition, Faculty of Medicine and Veterinary Medicine, Universitas Nusa Cendana, Kupang, Indonesia, Email: cynthia.gaina@staf.undana.ac.id
Online published on 11 July, 2023.
As one of the important genetic resources for Ongole beef cattle, the study aimed to identify any potential polymorphisms in the Bone Morphogenetic Protein 15 gene in a group of Sumba Ongole (SO) cattle.
Blood samples from 161 SO cattle were obtained and examined. The initial stage was the isolation of deoxyribonucleic acid (DNA). The next procedures were electrophoresis and extraction, followed by annealing temperature optimization, amplification and sequencing. The Basic Local Alignment Search Tool (BLAST) was used to examine the sequencing findings. The annealing temperature was 58°C for 15 seconds, with an amplification gain of 350 base pairs (bp).
The result showed that there were six single nucleotide polymorphisms (SNPs) of the BMP 15 gene in this region. Six mutations were SNP c.57 G>A, SNP c.120 C>T, SNP c.338 G>T, SNP c.360 C> A, SNP c.361 G>T, SNP c.365 A>T. The polymorphic informative content (PIC) value (0.25<=PIC<=0.5) was reached from low (0.02) to moderate high (0.45 and 0.47). A total of 2 SNPs in the BMP 15 gene with moderate-high PIC value was c.360 C>A and c.365 A>T. In conclusion, we reported for the first time the polymorphism of the BMP 15 gene which is required for further investigation as to whether this polymorphic could be responsible for reproductive trait variations in Ongole cattle.
BMP 15, Bos indicus, Cattle, Gene polymorphism, Reproductive, Sumba ongole
