Agricultural Science Digest

UGC CARE (Group 1)
  • Year: 2023
  • Volume: 43
  • Issue: 5

Optimization of pre-treatment incubation period on callus induction response in anthers of selected rice genotypes

  • Author:
  • P. Sharmela1, N. Meenakshi Ganesan1,*, R. Saraswathi2, R. Gnanam3, C.N. Chandrasekhar4
  • Total Page Count: 6
  • Page Number: 616 to 621

1Department of Genetics and Plant Breeding, Tamil Nadu Agricultural University, Coimbatore-641 003, Tamil Nadu, India

2Department of Plant Genetic Resources, Tamil Nadu Agricultural University, Coimbatore-641 003, Tamil Nadu, India

3Department of Plant Molecular Biology and Bioinformatics, Tamil Nadu Agricultural University, Coimbatore-641 003, Tamil Nadu, India

4Directorate of Open and Distance Learning, Tamil Nadu Agricultural University, Coimbatore-641 003, Tamil Nadu, India

Abstract

The microspores of five tropical japonica and five indica rice genotypes were subjected to androgenic studies. The effect of growth regulators on callus induction were studied to improve the anther culture efficiency.

The cold pre-treatment of panicles at 10°C were done at different days of intervals viz., 5, 8, 10 and 12 days. The microspores at uninucleate stage were selected and dusted after pre-treatment. The anthers were cultured in N6 basal media supplemented with casein hydrolysate (250 mg/L), proline (250 mg/L), silver nitrate (100 mg/L), maltose (50 g/L) and growth regulators.

The 8 days of cold pre-treatment initiated calli in most of the ten genotypes. The days taken for callus induction varied with genotype from 32-55 days. The callus induction frequency ranged from 1.41 to 5.12%. The responsive genotypes (Azucena, Palawan, Nira) on callus induction were studied for their regeneration potential.

Keywords

Anther culture, Callus initiation, Cold pre-treatment, Indica, Plantlet regeneration, Tropical japonica