1Division of Biotechnology, Nuziveedu Seeds Ltd., Gundlapochampally, Ranga Reddy, Hyderabad-501 401, Telangana, India
2Department of Biotechnology, Vignan's Foundation for Science Technology and Research, Deemed to be University, Vadlamudi-522 213, Guntur, Andhra Pradesh, India
*Corresponding Author: Satish Kumar Puligundla; Koigoora Srikanth, Division of Biotechnology, Nuziveedu Seeds Ltd., Gundlapochampally, Ranga Reddy, Hyderabad-501 401, Telangana, India, Email: puligun@yahoo.com
**Department of Biotechnology, Vignan's Foundation for Science Technology and Research, Deemed to be University, Vadlamudi-522 213, Guntur, Andhra Pradesh, India, koigooras@gmail.com, Orcid id: 0000-0002-8547-4680
Online published on 6 November, 2023.
This study has focussed on identifying the Simple Sequence Repeat (SSR) markers associated with the sucking pests (more precisely on jassid) tolerance/resistance loci. The study was initiated with 4348 mapped SSR markers to differentiate between resistant (R-1) and susceptible (BS-1) genotypes. Out of 4348 markers (retrieved from cotton microsatellite database and cottongen.org) used, 17 markers were showing polymorphism between the parental lines.
The F1 population was generated by crossing Resistant line (R-1) and the elite Susceptible line (Bs-1). F1 progeny were selfed to obtain F2 population. Following the screening, to identify the SSR markers associated with trait, Single Marker Analysis was performed.
The results indicated that two of the seventeen polymorphic markers - BL1646 and DOW047 had significant LoD of 4.5 and 7.2 respectively and PVE% of 6.6 and 10.3 respectively. Hence, these were identified as the two markers associated with jassid resistance trait having the potential for their use in marker assisted selection (MAS).
Cotton crop, Jassid resistance, Phenotyping, SSR markers
