Agricultural Science Digest
SCOPUS
  • Year: 2025
  • Volume: 45
  • Issue: 3

Optimized In vitro Propagation and Somatic Embryogenesis of Camu Camu (Myrciaria dubia) Cultivar Vitahuayo for Enhanced Commercial Production

  • Author:
  • M. Pedro Adrianzén1,2,*, F. Sergio Pinedo3, S. Barbara Valles2, L. Jorge Marapara2, Marianela Cobos1,2, N. Hicler Rodríguez1,2, C. Juan Castro1
  • Total Page Count: 7
  • Page Number: 468 to 474

1Specialized Unit of Biotechnology Research Laboratory, Natural Resources Research Center of UNAP, Universidad Nacional de la Amazonía Peruana (UNAP), Iquitos16001, Peru

2Academic Department of Biomedical Sciences and Biotechnology, Faculty of Biological Sciences, Universidad Nacional de la Amazonía Peruana (UNAP), Iquitos16001, Peru

3San Roque Agricultural Experiment Station, Direction of Genetic Resources and Biotechnology, Instituto Nacional de Innovación Agraria (INIA), Iquitos16001, Peru

*Corresponding Author: Pedro M. Adrianzén, Specialized Unit of Biotechnology Research Laboratory, Natural Resources Research Center of UNAP, Universidad Nacional de la Amazonía Peruana (UNAP), Iquitos16001, Peru, Email: pedro.adrianzen@unapiquitos.edu.pe

Online published on 04 December, 2025.

Abstract

Myrciaria dubia “camu-camu” a fruit-bearing shrub native to the Amazon, is known for its high vitamin C content. However, variations in vitamin C biosynthesis and accumulation among different cultivars present challenges for commercial production. This study aimed to establish an efficient in vitro regeneration protocol for the Vitahuayo cultivar through callus induction and somatic embryogenesis.

Stem and leaf explants were cultured on Murashige and Skoog (MS) medium supplemented with 24-dichlorophenoxyacetic acid (24-D) and 6-benzylaminopurine (BAP) to induce callus formation. Somatic embryos were induced using Woody Plant Medium (WPM) supplemented with 2,4-D, thidiazuron (TDZ), indole-3-butyric acid (IBA), naphthaleneacetic acid (NAA) and kinetin (KIN).

The best results for callogenesis were obtained with 1 mg/L of 24-D and 0.5 mg/L BAP. Somatic embryos were successfully induced with treatments T2 (2,4-D at 3 mg/L, NAA at 20 mg/L and KIN at 15 mg/L) showing 90% efficiency, while T3 (KIN at 0.3 mg/ L and BAP at 0.05 mg/L) achieved 57.5% effectiveness. These findings offer great potential for the large-scale propagation of Vitahuayo, the optimization of its commercial production and the standardization of vitamin C biosynthesis.

Keywords

Ascorbic acid, Callus induction, Plant regeneration, Somatic embryogenesis