1Department of Pharmaceutics, Satara College of Pharmacy, Satara-415 004, Maharashtra, India
2Department of Pharmaceutics, Laddhad College of Pharmacy, Yelgaon Buldana-443 001, Maharashtra, India
3Dr Balasaheb Vikhe Patil Rular Medical College, Loni-413 736, Maharashtra, India
*Corresponding Author: Arati Patil, Department of Pharmaceutics, Satara College of Pharmacy, Satara-415 004, Maharashtra, India, Email: arati.patil1690@gmail.com
Online published on 20 January, 2026.
In the present paper, Nano-Hydroxyapatite (n-HAp) is synthesized from Indian Hen (Busra breed) and Hybrid Hen eggshell waste using chemical precipitation methods. The principal objectives of this research are to synthesize natural HAp from eggshell waste and comparing with commercial available Hydroxyapatite (HAp) as reference sample (NnanoXIM-Hap-202).
Analytical methods were used to characterize n-HAp and HAp including Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive Spectroscopy (EDS), Differential Scanning Calorimetry (DSC) and Thermogravimetry-Differential Thermal Analysis (TG-DTA). Calcinations of eggshell waste at 850 to 900°C yielded HAp with a calcium/phosphorus ratio of 1.71 to 1.75.
A comparative analysis with a reference sample (commercially available HAp) revealed that the synthesized HAp exhibited similar structural and chemical properties, with slight variations in their morphological and thermal characteristics.
Bone graft, Calcium-phosphorus ratio, Eggs shell, Hydroxyapatite, Nanoparticles