1Department of Pharmaceutics, Satara College of Pharmacy, Satara-415 004, Maharashtra, India
2Department of Pharmaceutics, Laddhad College of Pharmacy, Yelgaon, Buldana-443 001, Maharashtra, India
3Department of Pharmaceutical Chemistry, Dr. D.Y. Patil Institute of Pharmaceutical Sciences and Research, Pune-411 018, Maharashtra, India
4Department of Pharmacology, Dr. D.Y. Patil College of PharmacyAkurdi, Pune-411 044, Maharashtra, India
*Corresponding Author: Arati Patil, Department of Pharmaceutics, Satara College of Pharmacy, Satara-415 004, Maharashtra, India, Email: arati.patil1690@gmail.com
Online Published on 16 April, 2026.
The poultry industry generates vast amounts of waste, including eggshells, which can be utilized as a source of nanohydroxyapatite (nHAp). This sustainable approach can reduce waste disposal costs, minimize environmental pollution and provide valuable biomaterials for various applications.
Indian Hen eggshells, hybrid Hen eggshells and a commercially available nHAp sample (as a reference) were used in this study. The biologically derived nHAp samples were synthesized via a precipitation method and the antioxidant activity and cytotoxicity of the nHAp samples were evaluated via the DPPH (1,1-Diphenyl-2-picrylhydrazyl) assay and MTT (Cell sensitivity) assay, respectively.
The results of the DPPH assay revealed that 3.125 μg/mL ascorbic acid was equivalent to 250 μg/mL Indian Hen eggshell-derived nHAp (IE1), whereas 1.56 μg/mL ascorbic acid was equivalent to 50 μg/mL hybrid Hen eggshell-derived nHAp (HE1). The reference sample showed that 0.78 μg/mL ascorbic acid was equivalent to 1 μg/mL ascorbic acid. The MTT assay revealed that all three nHAp samples were noncytotoxic and presented varying IC50 values (IE1: 1797 μg/mL, HE1: 1348 μg/mL, reference: 1972 μg/mL), indicating potential suitability for biomedical applications.
Antioxidant activity, Bone tissue engineering, Cytotoxicity, Hybrid hen eggshells, Indian hen eggshells, Nanohydroxyapatite
