Agricultural Science Digest
SCOPUS
  • Year: 2026
  • Volume: 46
  • Issue: 1

Nephrotoxicity of Iron Oxide Nanoparticles in Male Mice

  • Author:
  • Rawa Faris Hussein Al-Saeedi1, Raghad Khalid Mwafaq1, Noor Adel Jasim2, Zina F.H. Al-Obaidi3, Ahmed Flayyih Hasan4,5,*, Hany M. El-Wahsh6
  • Total Page Count: 6
  • Page Number: 38 to 43

1College of Science, AL Karakh University of Sciences, Baghdad, Iraq

2Department of Forensic Evideetnce, College of Science, Al-Nahrain University, Baghdad, 10072, Iraq

3Department of Medical and Molecular Biotechnology, College of Biotechnology, Al-Nahrain University, Baghdad, Iraq

4Biotechnology Research Center, Al-Nahrain University, Baghdad, Iraq

5Department of Biology, Al-Farabi University College, Baghdad, Iraq

6Department of Marine Biology, Faculty of Marine Sciences, King Abdulaziz University, Saudi Arabia

*Corresponding Author: Ahmed Flayyih Hasan, Biotechnology Research Center, Al-Nahrain University, Baghdad, Iraq, Email: ahmed_flayyih@nahrainuniv.edu.iq

Online Published on 16 April, 2026.

Abstract

The current study was conducted at Al-Nahrain University’s Biotechnology Research Center. This study’s objectives were to synthesize and characterize iron oxide nanoparticles (Fe2O3-NPs) and investigate the effects of varying concentrations on lipid peroxidation, the antioxidant defense system, biochemical parameters and kidney histology in male mice.

Twenty male Albinos Mice weighing between 25 and 30 g were utilized. The mice were split up into four groups of ten. Group II, Group III and Group IV received oral treatment with Tin oxide NPs at doses of 10, 25 and 50 mg/kg BW/day for four weeks, whereas the first group served as a control. Blood and kidney samples were taken for the examination of various parameters at the conclusion of the experiment.

Creatinine and urea levels dramatically rose following treatment with iron oxide nanoparticles (Fe2O3-NPs) at varying doses. Treatment with iron oxide nanoparticles (Fe2O3-NPs) at varying concentrations resulted in a significant decrease in the activities of AST, ALT and ALP in mouse kidney homogenates, as well as a significant increase in lactate dehydrogenase (LDH). Histopathological changes were noted, confirming the biochemical perturbations caused by iron oxide nanoparticles (Fe2O3-NPs) at varying concentrations in mice kidney.

Keywords

Fe2O3-NPs, Kidney histopathology, Liver, Mice, SOD, TBARS, Urea