1Department of Biology, Faculty of Nature and Life Sciences, University of Tiaret, Tiaret14000, Algeria.
2Laboratory of Improvement and Valorization of Local Animal Productions, Institute of Veterinary Sciences,University of Tiaret, Tiaret14000, Algeria.
3Department of Nutrition and Agri-food Technology, Faculty of Nature and Life Sciences, University of Tiaret, Tiaret14000, Algeria.
4Department of Ecology and Environmental Engineering, University of 8 Mai 1945, Guelma, Algeria.
5Institute of Veterinary Sciences, University of Tiaret, Tiaret14000, Algeria.
*Corresponding Author: S. Boudali, Department of Biology, Faculty of Nature and Life Sciences, University of Tiaret, Tiaret14000, Algeria. Email: souad.boudali@univ-tiaret.dz
The purpose of this study is to investigate the effects of the interaction between environmental pollutants and a high-fat diet on oxidative balance and intestinal microbiota composition. The research focuses on chronic exposure to low doses, equivalent to the acceptable daily intake (ADI), of bisphenol A (BPA), or tebuconazole (TEB), both incorporated into a high-fat diet.
Thirty female Wistar rats were allocated into six groups: Groups 1 and 2, which served as control groups, were not exposed to pollutants and were fed a standard diet (STD) and a high-fat diet (HFD), respectively. Groups 3 and 4 were exposed to bisphenol A (BPA), with group 3 receiving a standard diet (BPA) and group 4 a high-fat diet (BPAHFD). Groups 5 and 6 were exposed to tebuconazole, with group 5 receiving a standard diet (TEB) and group 6 a high-fat diet (TEBHFD), oxidative parameters in plasma and liver and microbial analyses were conducted.
After 16 weeks of experimentation, the findings revealed a negative impact on the oxidative balance of rats on HFD combined with BPA or TEB, as evidenced by an 89% increase in hepatic malondialdehyde (MDA) in the TEBHFD group compared to the BPAHFD group, along with a 30% reduction in plasma reducing power and a 47% reduction in liver reducing power. Furthermore, this treatment altered the intestinal flora composition of exposed rats, resulting in a 5% increase in enterobacteria. To this end, these data are sufficiently compelling to implicate TEB, even at tolerable doses, in the development of health issues and the exacerbation of oxidative stress and intestinal dysbiosis. The effect of TEB appears to be more pronounced than that of BPA.
Bisphenol A, Chronic toxicity, Intestinal microbiota, Oxidative stress, Tebuconazole, Wistar rat
