Bio-nano Synthesis Optimization of Lysinibacillus sp as Biostimulant and Biocontrol to Increased Rice Plants Production

The increasing demand for rice production in Indonesia necessitates innovative and sustainable approaches to enhance crop yield while minimizing environmental impact. Excessive use of chemical fertilizers and the prevalence of plant diseases remain major challenges in rice cultivation. This study aims to optimize the bio-nano synthesis of Lysinibacillus sp. for its potential application as a biostimulant and biocontrol agent to improve rice productivity.

Silver nanoparticles (AgNPs) were biosynthesized using Lysinibacillus sp. cultivated in different growth media. The synthesized AgNPs were characterized and evaluated for their biocontrol activity against major rice pathogens, including Xanthomonas oryzae and Rhizoctonia solani. In addition, the phytohormone-producing ability of Lysinibacillus sp. isolates was assessed through qualitative indole-3-acetic acid (IAA) detection using the Salkowski reagent.

The optimized bio-nano synthesis process successfully produced AgNPs with significant biocontrol activity against Xanthomonas oryzae and Rhizoctonia solani. AgNPs synthesized in luria bertani (LB) medium at 50% concentration exhibited the highest inhibition zones, indicating strong biocontrol potential. Furthermore, Lysinibacillus sp. isolates demonstrated the ability to produce IAA, a key phytohormone involved in plant growth promotion. These results highlight the potential of Lysinibacillus sp. as an effective biostimulant and biocontrol agent for sustainable rice production.