Baba Farid University Dental Journal

  • Year: 2023
  • Volume: 13
  • Issue: 2

Comparison of bacterial removal from dentinal tubules with two different bacterial biosurfactants and a herbal extract: An in vitro confocal laser scanning microscopic study

1Consultant Endodontist, Ex-Post Graduate, Department of Conservative Dentistry and Endodontics, Sri Guru Ram Das Institute of Dental Sciences and Research, Amritsar, Punjab, India

2Professor& Head of Department Department of Conservative Dentistry and Endodontics, Sri Guru Ram Das Institute of Dental Sciences and Research, Amritsar, Punjab, India

Abstract

This study compared various experimental endodontic irrigants for their ability to kill Enterococcus faecalis in dentinal tubules.

The root canals of 55 extracted single-rooted incisors were prepared. The teeth were autoclaved and inoculated with E. faecalisfor 21 days. The infected samples were then randomly divided into 5 experimental groups of 10 teeth each and a control group of 5 teeth as follows: group 1: 5% sodium hypochlorite solution (NaOCl); group 2: 2% chlorhexidine; group 3: 5% Withania somniferaextract; group 4: Bacillus subtilisbio-surfactant ; group 5:Pseudomonas aeruginosabio-surfactant; and group 6: distilled water. After the experimental period, the specimens were stained with fluorescein diacetate (FDA) and propidium iodide (PI) dyes. Statistical analyses were performed using the one way ANOVA and Tuckey post hoc tests.

The percentages of dead cells were highest in the Bacillus subtilisgroup, amongst all the experimental irrigants.

Bio-surfactants of Bacillus subtilisand Pseudomonas aeruginosaproved to be superior in terms of viability of enterococcus faecalis and have the potential to be used as intracanal irrigants.

Keywords

Withania somnifera, Biosurfactant, Enterococcus faecalis, Intracanal irrigants