*Departmat of Biotechnology, Manav Bharti University, Kumarhatti-Solan-173229, Himachal Pradesh
Department of Biotechnology, Dr. Y. S. Parmar University of Horticulture and Forestry, NauniSolan-173230, Himachal Pradesh
Online published on 28 February, 2013.
Randomly amplified polymorphic DNA (RAPD) was used to investigate genetic relationships among 25 genotypes of cauliflower (Brassica oleracea L. var. botrytis). Aset of twenty random primers were examined, among which 12 primers produced polymorphism. Sixty-eight bands were obtained, ranging in size of the amplified DNA segments from 72 bp to 2440 bp, out of which 55 were polymorphic and 13 were unique. Genetic similarity matrix was computed on the basis of Jaccard's coefficients, which indicated wide genetic base among cauliflower genotypes. Dendrogram was constructed by UPGMA method. RAPD analysis provided quick and reliable alternative for the identification of cauliflower genotypes and for determination of the relationships among them.
Brassica oleracea L, Cauliflower, RAPD, genetic diversity, molecular markers
