Effect of passage on the development of aliette resistance in damping off of radish

Exposure of wild sensitive isolate of Pythium aphanidermatum (Pa-3) to aliette, continuously for 8 successive passages increased resistance in both in vitro and in vivo conditions. During in vitro studies, when aliette was altered with blitox, there was complete inhibition of the pathogen at II^nd passage, and at III^rd passage in case of dhanuka and carbendazim. During in vivo conditions, when aliette was altered with blitox, disease was completely managed at II^nd passage, at V^th passage in case of dhanuka and VII^th in case of carbendazim. There was complete inhibition of the pathogen at II^nd passage in case of dhanuka and blitox and at passage III^rd in case of carbendazim. The results are of great importance in management of Damping off of radish.