Detection of genetic variation in brinjal shoot and fruit borer (Leucinodes orbonalis G.) populations using rapd markers

Genomic DNA of ten different populations of Leucinodes orbonalis G. from North Karnataka were analysed for studying genetic variation among them. Fourty decamer primers were used in this analysis, which generated a total of 244 markers revealing an average of 14.35 markers per primer in each population. Genetic relationships between the populations were evaluated by generating similarity matrix (based on Jaccard's index) and phonetic dendrogram was generated (by UPGMA method). Principal component analysis separated 12 populations into different groups based on band-sharing data. Populations showed varied degrees of genetic similarity within the range of 0.04 -0.52.