BIOINFOLET - A Quarterly Journal of Life Sciences
Web of Science
  • Year: 2013
  • Volume: 10
  • Issue: 4c

Isolation, screening and antagonium assay of pseudomonas spp. for plant growth propoting activity and its campatibility with pesticide molucules

  • Author:
  • J. Sai Prasad, Manish Jha, R. Naveen Kumar, Arbind Kumar Gupta
  • Total Page Count: 5
  • Page Number: 1487 to 1491

*Department of Plant Pathology, College of Agriculture, Rajendranagar, Acharya N G Ranga Agricultural University, Hyderabad-500 030, Andhra Pradesh, India

**Department of Soil science and Agricultural Chemistry, College of Agriculture, Rajendranagar, Acharya N G Ranga Agricultural University, Hyderabad-500 030, Andhra Pradesh, India

Department of Agricultural Microbiology and Bioenergy, College of Agriculture, Rajendranagar, Acharya N G Ranga Agricultural University, Hyderabad-500 030, Andhra Pradesh, India

Online published on 19 December, 2013.

Abstract

Twenty six isolates of plant growth promoting Pseudomonas spp. were isolated and identified. They were in vitro screened for PGPR properties like phosphate solubilization, siderophore, IAA, HCN productions, antagonistic activity against Rhizoctonia solani, Sclerotium rolfsii and compatibility with commonly used pesticide molecules. The results revealed that all Pseudomonas isolates were positive for IAA production, 76.9% for phosphate solubilization, 92.3% for ammonia, 88.46% for siderophores and 80.76% for HCN productions. Eight isolates showed inhibition potential against Rhizoctonia solani and Sclerotium rolfsii. Pseudomonas isolates CBuP1, CBpP1, CBpP2, SBuP2, ABuP1, ARpP2 and AmaP1 could be considered for their bio control activity. Among the pesticides tested, Azoxystrobin (fungicide), Flubendiamide (insecticide) and Pretilachlor (herbicide) inhibited growth of Pseudomonas. However, other fungicides, insecticides and herbicides were compatible with all of the isolates tested. Isolate of Pseudomonas isolate ARuP1 showed all properties of PGPR.

Keywords

Pseudomonas, Biochemical characterization, PGPR, Biocontrol, compatibility