Department of Plant Biotechnology, UAS (B), GKVK, Bangalore-65
Online published on 1 May, 2014.
Cucumber (Cucumis sativus L.) cv. Green Long was used for callus regeneration from cotyledon and leaf explants. Significantly high amount of callus (1.418g) was obtained from cotyledon explants in a combination of 1μM 2, 4-D and 4μM BAP, which was green, compact and better than that in all treatments of leaf explants. Cucumber Mosaic Virus (CMV) was successfully multiplied in cucumber callus which was confirmed through Dot-Enzyme Linked Immunosorbent Assay (ELISA). Calli derived from both explants of cucumber were transformed with gus gene using gene gun.
Cucumis sativus L, callus induction, multiplication of CMV, ELISA, Gus gene transformation
