Development of regeneration system in tomato (Solanum lycopersicum L.)

In vitro regeneration of tomato (Solanum lycopersicum L.) was attempted using shoot tip and hypocotyl explants. The MS medium supplemented with zeatin (2 mg/l) and IAA (0.2 mg/l) was found to be effective for direct shoot induction and plantlet regeneration, with maximum number of shoots per explant. Highest shoot regeneration frequency was recorded with shoot tip explant (89.60%) followed by hypocotyl explant (88.27%). Number shoots per explant was high (7.13) in hypocotyl explant. Maximum rooting (86.64%) was observed without growth regulators.