BIOINFOLET - A Quarterly Journal of Life Sciences
Web of Science
  • Year: 2015
  • Volume: 12
  • Issue: 1B

Validation of scar markers for rust resistance in french bean (Phaseolus vulgaris L.)

  • Author:
  • Soumya Shetty, K.V. Ravishankar, T. S. Aghora
  • Total Page Count: 8
  • Page Number: 154 to 161

*Division of Vegetable crops, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore-560089, India

Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore-560089, India

Online published on 17 March, 2015.

Abstract

Three resistant (IIHR-79, IIHR-31 and Arka Anoop) and two susceptible (1IHR-55 and Arka Suvidha) parents along with F2 cross IIHR-31X IIHR-55 were used to identify marker linked to rust resistance. A total of 10 SCAR primers (SK14, SA14, S119, SBC6, SAD12, SAE19, UR11-GT2, KB126, SF10 and SBA8) were used for screening of the contrasting parents so as to get polymorphism for rust resistance. Out of 1 0 selected SCAR markers, SA-14, SK14 and SF-10 showed polymorphism between resistant and susceptible parents. For the markers, SA14800 and SK14620 polymorphic band was observed in resistant parent IIHR-31. Whereas, for the marker SF101072 polymorphic band was observed in resistant parent IIHR-79. Identified markers SA-14800 and SK14620 were again validated in F2 cross IIHR-31X IIHR-55.

Keywords

Bulk segregant analysis, Rust, Phaseolus vulgaris L, SCAR marker