*Biotechnology centre, Department of Agricultural Botany, Dr. PDKV, Akola, Maharashtra
**Vasantrao Naik Marathwada Krushi Vidyapith, Parbhani, Maharashtra
V. D. College of Agriculture Biotechnology, Latur, Maharashtra
Online published on 17 March, 2015.
Highest callus induction frequency was observed in hypocotyl (86.40%) followed by leaf bit (78%) on MS basal medium containing 5 mg/I NAA and 0.5 mg/I BAP. The callus was subjected to desiccation using silica gel and filter paper. Desiccation percentage were 11.78, 18.00, 22.85 and 28.26% in silica gel, where as with filter paper it was 44.53,49.82, 52.92 and 59% for two, four, six and eight hour of desiccation respectively. These desiccated callus may be sub-cultured on proliferation media.
Cotton, Callus culture, desiccation, silica gel
