Identification of molecular markers for fertility restoration genes in rice (Oryza sativa L.)

Present investigation was undertaken on mapping of fertility restorer gene in F₃ population of IR58025AxIR72164-352-2-5-5 with the help of micro satellite markers. Among F₃ population, line number 52 had high pollen and spikelet fertility, while line number 3 (0.25) showed lowest pollen and spikelet fertility, these lines may be considered as suitable for restorer and maintainer lines respectively. Four DNA markers, across 2 chromosomes (Chr1 and 10) reported to be closely linked with Rf genes. RM490 linked with Rf₃ gene was located on short arm of chr1 with map distance of 2.8cM; RM6100 and RM1108 were located on Chr1 0 with map distance of 1.2cM and 1.5cM, respectively from R~gene. Rfgenes of the SSR markers reported in this study might be useful for the identification of CMS lines of rice through marker-assisted selection with reasonable accuracy.