Department of Livestock Products Technology, College of Veterinary Science and Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, S.K. Nagar, 385–506
Online published on 3 December, 2016.
Multiplex PCR assay was quick, simple and sensitive method for identification multiple meat species in single PCR reaction. Mitochondrial cytochrome b gene was used as an indicator gene for identification of selected animal species (Buffalo, Sheep, Goat and Chicken) by using multiplex PCR. Sensitivity of the multiplex PCR assay was determined by using 100 ng/μl DNA from meat from the samples of these animals. During present study the sensitivity of multiplex PCR assay was fined up to 0.01 ng/μl DNA.
Multiplex PCR assay, Primer Mixture, sensitivity, Cytochrome bgene
