BIOINFOLET - A Quarterly Journal of Life Sciences
Web of Science
  • Year: 2017
  • Volume: 14
  • Issue: 3

Preparation and storage of bioinoculants from azospirillum brasilence (Dobereiner, 1978), Bacillus megaterium (Ehrenberg, 1835) Var. phosphaticum and pseudomonas fluorescens (Migula, 1985)

  • Author:
  • M. Jeya Bharathi, D. Balachandar1, K. Kumar2
  • Total Page Count: 4
  • Page Number: 316 to 319

1Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore

2Natural Resource Management, Tamil Nadu Agricultural University, Coimbatore

Department of Plant Pathology, Tamil Nadu Rice Research Institute, Aduthurai-612101

Online published on 26 December, 2017.

Abstract

Liquid bio-inoculant's were prepared using Nitrogen fixing Azospirillum brasilense, Phosphorus solubilising Bacillus megaterium var. phosphaticum and plant growth promoting rhizobacteria Pseudomonas fluorescens. The results obtained indicated that the inoculants supplemented with 10 mM of phosphate buffer (pH 7) + 10% glycerol supported maximum survival of Azospirillum (0.17 x 1019 MPN/mL), Bacillus (25 x 1019 cfu/mL) and Pseudomonas (21 x 1019 cfu/mL) population at room temperature.

Keywords

Nitrogen fixing bacteria, Phosphorus solubilizing bacteria (PCB), plant growth promoting rhizo-bacteria (PGPR), cell protectants, storage