Present study revealed the effect of metal ions on the production of enzyme amylase by four fungi viz Aspergillus niger, Helminthosporium oryzae, Aspergillus flavus, Curvularia lunata and Fusarium oxysporum, which were cultivated on deproteinized leaf extracts of Medicago sativa, Spinacia oleracea, Anethum graveolens and Trigonella foenium-graecum. Maximum amylase production was achieved by Aspergillus niger. The production of amylase increased due to the addition of ZnSo4 to the deproteinized leaf extract of Medicago sativa, when Aspergillus niger was cultivated on it.
Amylase, Lucerne, DPJ
