Serum enzyme changes associated with heat stress in buffaloes

Eighty buffaloes of Nagpuri breed were kept under observation from February to June 2009 to 2010 and blood parameters were observed fortnightly. Blood serum samples were analysed for the activities of Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), alkaline phosphatase (ALP) by using commercial kits. The activities of aspartate amino transferase and alanine amino transferase were higher under heat stress conditions, mainly due to their leakage from liver cytosol into the blood stream, which reflects liver damage and disruption of normal liver function.