Determination of composition and stability constant of cu (ii)-proline and cu (ii)-histidine in aquo-organic medium

Exhaustive work was carried out on the comlexometric study of amino acids in aquo-organic medium. The equilibrium behavior of the amino acids has been studied spectrophotometrically in the presence of Cu (II) ion in aquoorganic and at isodielectric constant medium. The molar extinction coefficient was determined from the slope of absorbance vs concentration of complex solution. It was also predicted from ratio of absorbance to the concentration of complex used. In order to evaluate formation constant again different composition (M:L) were prepared and measurements were made spectrophotometrically.