Isolation of protease inhibitor from the leaves of Terminalia arjuna and Terminalia cattapa

Present investigation was undertaken on estimation of Protease inhibitor activity (PI) from the leaves of Terminalia arjuna and T. cattapa. The results revealed 80- 85 % and 60-70 % protease inhibitor activity in the leaves of T. arjuna and T. cattapa respectively. Maximum Protease inhibitor activity was detected in the months of November and December (2018). The enzyme was subjected to Sephadex G50 purification. The samples analyzed on SDS PAGE revealed a 30kDa size of protein with Protease inhibitor (PI) activity.