BIOINFOLET - A Quarterly Journal of Life Sciences
Web of Science
  • Year: 2021
  • Volume: 18
  • Issue: 1b

Tlc-Bioautography and gc-ms of endophyte Cladosporium cladosporioides isolated from Citrus limon

  • Author:
  • Manisha Survaseand Santosh Taware
  • Total Page Count: 6
  • Page Number: 171 to 176

Mahatma Gandhi Mission, Institute of Biosciences and Technology, Aurangabad, Maharashtra, India

Online published on 27 April, 2021.

Abstract

Cladosporium cladosporioides was isolated from leaves of Citrus limon. Agar well diffusion assay of Cladosporium cladosporioides showed maximum zone of inhibition against Alternaria alternata than Fusarium oxysporum, Trichoderma viride and Penicillium notatum. Thin layer chromatography-bioautography and gas chromatography-mass spectrometry revealed a single spot (Rf value 0.28) as a mixture of N-Hexadecanoic acid, 9-Octadecenamide and squalene to be the antifungal molecules in the extract, inhibiting the growth of Alternaria alternata.

Keywords

Citrus limon, Endophytic fungi, Pathogenic fungi, TLC, TLC- Bioautography