Effect of amino acids and vitamins on antagonistic activity of trichoderma viride

The antagonistic activity of Trichoderma viride was evaluated against Pythium aphanidermatum (Edson) Fitzp. causing turmeric rhizome rot by using Dual culture technique. To enhance antagonistic potential of this bio-agent physical and chemical mutagenesis of T viride was carried out by using UV light and Sodium azide (SA) respectively. From these, 2 mutants namely T_VUV-48 and T_vsa-56 were selected for present study. In the present investigation, effect of physiological factors such as amino acids and vitamins on antagonistic activity of three isolates of Trichoderma viride (T_VW T_VUV-48 and T_VSA-56) were determined Highest percent inhibition was recorded when lsoleucine(70.0%) was used in case of T_VS and Proline (72.2%) and Histidine (66.3%) for T_VUV-48 and T_VSA-56 respectively. Remarkable results for vitamins (Nicotinic acid, Riboflavin and Ascorbic acid) were recorded in case of T_VUV-48 among which Nicotinic acid was most effective for all Trichoderma viride isolates.