BIOINFOLET - A Quarterly Journal of Life Sciences

Web of Science
  • Year: 2021
  • Volume: 18
  • Issue: 4

Detection, characterization and identification of protease inhibitor (HVPI) from Hydrilla Verticillata (L.F.) royle

  • Author:
  • Sandip K. Wagh, Rahim A. Bagwan1, Ayesha G. Shaikh, Manohar V. Padul2, Abhay N. Salve
  • Total Page Count: 6
  • Page Number: 477 to 482

1Department of Botany, Government Institute of Science, Aurangabad, (Maharashtra), India

2Department of Biochemistry, The Institute of Science, Dr. Homi Bhabha State University, 15, Madame Cama Road, Fort, Mumbai-400032, MaharashtraIndia

Department of Biochemistry, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, (Maharashtra), India

Online published on 20 January, 2022.

Abstract

Protease inhibitors (Pis) are useful constituents in plants and animals, playing vital endogenous functions.Present investigation was undertaken on detection and characterization of protein inhibitors (Pis) from Hydrilla verticillata i.e. HVPI. The detection of HvPI was carried out by dot-blot assay, reverse zymography and Gel X-ray Contact Print (GXCP) using enzyme trypsin. Molecular weight of HvPI was found to be 23 kDa as determined by 10% SDS PAGE. The optimum pH and temperature for its activity was found to be 7 and 40°C respectively. MALDI-TOF mass spectrometric analysis of peptides showed sequence similarities with flavanone 3-hydroxylase, which is an essential enzyme of flavonoid biosynthesis. Additional investigation is needed to establish therapeutic potential of HvPI, as future prospective of this study.

Keywords

Hydrilla verticillata, Protease inhibitor, HvPI, GXCP, Therapeutic