Flow cytometry analysis of SH-SY-5Y cells treated with MN304 nanoparticles

Effect of Mn₃O₄nanoparticles on SH-SY-5Y neuroblastoma cell line was studied. SH-SY-5Y cell line was treated with different concentrations of Mn₃O₄ nanoparticles for 24 hours. Toxicity of Mn₃O₄ nanoparticles was quantified by MTT assay, Annexin Pi apoptosis assay, Cell Cycle Analysis, ROS assay and microscopic observations. It was observed that Mn₃O₄ nanoparticles are not toxic to SH-SY-5Y cell line at lower concentration. However decreased cell viability and cell death by necrosis at higher concentration of Mn₃O₄ nanoparticles proposes appropriate quantity of nanoparticles have potential in biomedical application.