1Department of Animal Biotechnology, ICAR-National Bureau of Animal Genetic Resources, Karnal, Haryana, India
Department of Computer Science and Applications, Baba Mastnath University, Rohtak, Haryana, India
Online published on 18 January, 2023.
As a tool for characterizing transcriptomes, RNA sequencing (RNAseq) has quickly gained popularity. In many RNAseq studies, identifying molecular markers plays a significant role. Though several data analysis pipelines and methods have been developed for differential gene expression (DGE) analysis, there is no clear consensus about best practices. An assessment of different tools used in the pipeline for DGE analysis was conducted during presentstudy.
RNA sequencing, Differential gene expression, RSEQREP, Alignment, Feature Counts, Quantification