Induced chemical and physical mutagens on plant survival and pollen fertility of grass pea (Lathyrus sativus L.).

The seeds of Grass pea (Lathyrus sativus L.) were treated with various doses of chemical mutagens [EMS (0.05, 0.10 and 0.15%); SA (0.05, 0.10 and 0.15%); NMU (0.15, 0.30 and 0.45%)] and physical mutagen (Gamma rays-15, 30, 45 GY). The per cent plant survival at flowering stage gradually decreased with the increase in the doses of chemical and physical mutagens. It was 68.35 to 38.74% in EMS (0.05 to 0.15 %); 69.57 to 32.67% in SA(0.05 to 0.15%); 71.02 to 38.91% in NMU (0.15 to 0.45%) and in Gamma Rays it was 70.45 to 37.95% (15 to 45 Gy) respectively. The impact of various doses of physical and chemical mutagens showed significant reduction in pollen fertility in M₁ generation over control.