Isolation and screening of amylase producing thermophiles from compost.

Although there are various biological sources of amylase enzyme, microbial source in the form of Bacillus sp., is widely preferred, amongst amylase producing bacteria. In this respect B. subtilis, B. amyloliquifaciens and B. licheniformis are most commonly employed bacterial strains for amylase production (Lestari et al., 2012). Present study reports isolation of amylase producing thermophiles from compost. The suitable isolates were thereafter screened for amylase production. The suitable isolate was then subjected to 16 r RNA gene sequencing and blast analysis, through which it was identified as the strain of B. subtilis subsp subtilis.