In vitro propagation of the medicinal plant Abrus fruticulosus L.

A protocol was developed for in vitro propagation of Abrus fruticulosus L. through induction of organogenesis in callus tissues derived from nodal segments. Yellowish green nodular callus was cultured on MS medium fortified with 5.0 mg/l BAP and 0.5 mg/l NAA. The callus differentiated into adventitious shoots, when it was sub-cultured on MS medium supplemented with 3.0 mg/l BAP +0.5 mg/l Kn +0.5 mg/l NAA. On an average 6.87 ± 0.26 shoots/culture were developed. These microshoots were rooted on MS media containing 1.0 mg/l IBA.