Callus was regenerated from slices of cormel sprouts of Gladiolus primulinus cv. Golden Wave, cultured on MS medium with 4.0 mg/I NAA, 2.0 mg/l BAP or 1.0 mg/l 2,4-D. Shoots were regenerated from the callus on MS medium with 0.5–2.0 mg/l Kn or/and 0.5–2.0 mg/l BAP. Highest number of shoots were regenerated with 2.0 mg/l BAP from one-month-old callus which was initiated in the medium with NAA. Shoot regeneration capacity of callus decreased with the age and after fifth month no shoot was regenerated. The shoots produced roots in the medium with 2.0 mg/l IBA and 6% sucrose. Rooted shoots produced cormels at the base in the same nutrient medium. About 80–90% cormels germinated when sown in the field and 20% of them blossomed in the first year.
Gladiolus, Ornamental, Regeneration, Carmel formation
