*Department of Botany, Shri Shivaji College of Arts, Commerce & Science, Akola-444 001 (M. S.)
Department of Microbiology, Shri Shivaji College of Arts, Commerce and Science, Akola-444 001 (M. S.)
Online published on 17 January, 2012.
Present paper deals with regeneration of Withania somnifera (L.) Dunal through nodal explants. The MS (Murashige and Skoog) medium, supplemented with 0.3 mg/l BAP (6-Benzyl Amino Purine) and 0.1 mg/l NAA (Naphthalene Acetic Acid) was used for induction of multiple shooting. Increasing concentration of BAP increased number of shoots per explant. Maximum numbers of shoots were observed with 2.0 mg/l BAP (6-Benzyl Amino Purine). The shoots were rooted with 0.3 mg/l IBA (Indole-3 Butyric Acid) containing media and transferred successfully to the field.
In vitro regeneration, nodal explants, Withania somnifera.
