Rapid HPLC Method for Determination of Cholesterol using C18 Column

A method for quantitative estimation of cholesterol using C18 reversed phase column and evaporative light scattering detector was developed by optimizing technical parameters, such as column oven temperature (45⁰C), mobile phase composition (acetonitrile - isopropanol, 60: 40), mobile phase flow rate (1.5 ml/min), nebulizer temperature (55⁰C) and evaporator temperature (85⁰C). The calibration curve was established with 2.5 - 160 µ/ml standard cholesterol with correlation coefficient (r²) value of 0.99.