Department of Biotechnology, University of Agricultural Sciences, Dharwad
Department of Agricultural Entomology, University of Agricultural Sciences, Dharwad
University of Agricultural Sciences, Raichur
Online published on 24 August, 2012.
Populations of Helicoverpa armigera collected from three hosts viz., cotton, chickpea and pigeonpea were screened using two cypermethrin resistance associated markers, GFPAT05300 and GFAB04500 The field collected H. armigera were assayed for their levels of resistance to cypermethrin insecticides. Later the DNA isolated from the same individuals were screened for the DNA marker status; resistant populations of all the three hosts showed markers positive. The results indicated that all the three populations recorded cent per cent fidelity for GFPAT05300 marker in all the three host strains and GFAB04500 marker recorded 89,47 and 87.5 per cent fidelity respectively in cotton and pigeonpea host strains.
Helicoverpa armigera, insecticide resistance, DNA marker, RAPD-PCR
