* Dept. of Biotechnology, College of Agriculture, Dharwad, University of Agricultural Sciences, Dharwad
**Dept. of Plant Pathology, College of Agriculture, Dharwad, University of Agricultural Sciences, Dharwad
***Dept. of Entomology, College of Agriculture, Dharwad, University of Agricultural Sciences, Dharwad
Dept. of Agricultural Microbiology, College of Agriculture, Dharwad, University of Agricultural Sciences, Dharwad
Online published on 24 August, 2012.
Fifty rhizobacterial isolates were screened against Tomato leaf curl Virus (ToLCV) disease under glasshouse condition. Application of rhizobacteria based bioformulations to seed, soil and foliage significantly reduced the disease severity of ToLCV from 28.58 to 85.72% with Pseudomonas sp 206 (4) and Pseudomonas sp. B-15. All isolates induced systemic resistance to the disease. The plants inoculated with Pseudomonas sp. 206 (4) recorded highest phenolic content, and peroxidase, polyphenol oxidase, chitinase and PALase activities with reduction in insect population. Semi quantitative PCR analysis revealed lower viral load in plants inoculated with these isolates. Electron microscopic examination of ToLCV revealed the presence of isometric and pentagonal Gemini virus, (20 × 30 nm to 24 × 30 nm). Treatment with fluorescent Pseudomonas 206(4) recorded maximum plant height, total biomass and chlorophyll content.
Pseudomonas spp, ToLCV, Tomato, Induced Systemic Resistance, Biocontrol, Bemisia tabaci
