Callus induction and secondary metabolites from Tribulus terrestris L. and Pedalium murex L.

Present paper deals with in vitro callus induction in two medicinal plants Tribulus terrestris L. and Pedalium murex L. from leaf explant cultured on MS medium supplemented with 2,4-D (0.5, 1.0, 2.0 mgl⁻¹), BAP (0.5,1.0,2.0 mgl⁻¹) and Kin ((0.5,1.0,2.0 mgl⁻¹). 2,4-D (2.0 mg/l⁻¹) exhibited 61.83% callus formation with leaf explants of T. terrestris while 52.30% with P. murex. Total alkaloids, flavonoids and phenolic contents of the leaf tissues were 1.8 times higher than that in the callus of T. terrestris. In case of P. murex it was 2.5 times higher.