BIOINFOLET - A Quarterly Journal of Life Sciences

Web of Science
  • Year: 2012
  • Volume: 9
  • Issue: 4b

Plant regeneration, genetic transformation and gene expression in In vitro tissues of cauliflower (Brassica oleracea L. var. Botrytis)

  • Author:
  • Sneh Sharma, Chhaya Sharma, D.K. Srivastava
  • Total Page Count: 5
  • DOI:
  • Page Number: 760 to 764

Department of Biotechnology, Dr. Y.S. Parmar University of Horticulture and Forestry, Solan-173230, Himachal Pradesh, India

*School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana-141001, Punjab, India

Abstract

Plant regeneration and Agrobacterium-mediated gene transfer studies were carried out in cauliflower (Brassica oleracea L. var. botrytis cv. Pusa Snowball K1). Higher frequency of shoot regeneration was obtained in cotyledon (68%) and hypocotyl (84%) explants on MS medium supplemented with 2.0 mg/l BAP + 0.5 mg/l IAA. Addition of 0.05 mg/l IBA in nutrient medium promoted regeneration of roots. Agrobacterium tumefaciens, strain LBA 4404 was used for genetic transformation studies. Transformed cells were found to be able to grow on selective medium. Successful genetic transformations were confirmed by β-glucuronidase enzyme assay.

Keywords

Cauliflower, plant regeneration, genetic transformation, Agrobacterium, binary vector, npt-II and gus genes, gene expression