*School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana-141001, Punjab, India
Department of Biotechnology, Dr. Y.S. Parmar University of Horticulture and Forestry, Solan-173230, Himachal Pradesh, India
Online published on 3 January, 2013.
Plant regeneration and Agrobacterium-mediated gene transfer studies were carried out in cauliflower (Brassica oleracea L. var. botrytis cv. Pusa Snowball K1). Higher frequency of shoot regeneration was obtained in cotyledon (68%) and hypocotyl (84%) explants on MS medium supplemented with 2.0 mg/l BAP + 0.5 mg/l IAA. Addition of 0.05 mg/l IBA in nutrient medium promoted regeneration of roots. Agrobacterium tumefaciens, strain LBA 4404 was used for genetic transformation studies. Transformed cells were found to be able to grow on selective medium. Successful genetic transformations were confirmed by β-glucuronidase enzyme assay.
Cauliflower, plant regeneration, genetic transformation, Agrobacterium, binary vector, npt-II and gus genes, gene expression
