National Dairy Research Institute, Karnal-132001.
Bovine Leukocyte Adhesion Deficiency Syndrome (BLAD) is inherited as autosomal congenital lethal recessive disorders. BLAD is spread throughout the world by use of carrier bulls in Artificial insemination. BlAD in Holstein cattle is characterized by recurrent bacterial infections, delayed wound healing, stunted growth and is associated with persistent marked neutrophilia. The molecular basis of BlAD is a single point mutation (adenine to guanine) at position 383 of the CD18 gene, which caused an aspartic acid to glycine substitution in the glycoprotein. Keeping this in view the present study was conducted to screen Karan Fries (KF) animals for single point mutation in CD18 gene. In this study, 10 ml biood was collected from 110 Karan Fries animals from Cattle Yard and Artificial Breeding Complex, National Dairy Research Institute, Kamal, Haryana (India). Genomic DNA was isolated by phenol ch10roform extraction method. The PCR was performed using primers already reported in previous studies. The PCR products obtained were subjected to RFLP analysis using Taq I restriction enzymes. The results of RFLP showed that 24 out of 110 animals were heterozygous and 2 were recessive homozygous for BlAD. The estimated frequencies of dominant and recessive alleles in the ~ screened population were 0.873 and 0.127 for BLAD. Frequencies of carrier and homozygous recessive animals (genotype) were 21.82 and 1.82 per cent, respectively. Considering results of this study, it is recommended that all the KF cattle at NDRI should be screened for BlAD. This also calls for screening of other population of HF and its crosses in the country.
