The present study was carried out to evaulate the antibacterial activity of the cold aqueous (CAE) and cold ethanolic (CEE) extracts of Carum copticum against bovine mammary pathogens viz., Staphylococcus aureus and E. coli isolated from mastitic milk samples. The growth inhibition zones for Staphylococcus aureus against the aqueous and ethanol cold extracts of Carum copticum were 21.66 ± 0.34 mm and 36.00 ±0.49 mm, repsectively and that for E. coli were 18.50 ± 0.86 mm and 31.33 ± 0.62 mm, respectively. The results suggested that the inhibition zones of aqueous extract Staphylococcus aureus were larger than E. coli. The CAE and CEE of Carum copticum exhibited antibacterial properties.