1ICAR, National Bureau of Animal Genetic Resources, Karnal, Haryana
ICAR-National Bureau of Animal Genetic Resources, Karnal, Haryana-132001, India
2Ph. D. Scholar, ICAR-NDRI, Karnal
3Regional Research Centre, Karnal
High-resolution melting (HRM) assay is a novel tool for polymorphism analysis. We describe here the application of HRM analysis for screening of two polymorphic sites on the ZFY gene (ZFY-9 and ZFY-10) present at male specific region of bull Y-chromosome which are generally being used to identify Y-chromosome haplotypes (Y1, Y2 and Y3) in many cattle breeds/population world wide. The Y-chromosome haplotypes are important for the understanding of effective male contribution, Y-chromosome diversity and male lineages. Wetested 20bulls with known genotypes for two sites(ZFY-intron 9, 120> C/T; and ZFY-intron 10, 655> C/T)that were previously genotyped by Allele Specific PCR (AS-PCR) and validated through direct sequencing (two representative samples from each region) and then examined the sensitivity of mutati on detection using quantitative PCR (qPCR) followed by HRM curve analysis. Absolute concordance was observed among AS-PCR, direct sequencing and HRM analysis. Known Y-chromosome
