The study was conducted to investigate the effect of lameness associated pain and stress on semen quality in crossbred Frieswal bulls. Sixteen bulls which developed lameness due to ulcers, blisters, wounds etc in interdigital space, coronet and around hooves were selected for the study. Semen samples were collected by AV technique twice a week from each bull during one month before and two month after development of lameness. The fresh ejaculates were subjected to evaluation for volume, sperm concentration, initial motility, post thaw motility as per standard procedures. The clinical signs observed in the affected bulls were dullness, depression, limping of the affected leg, disinterest in the mounting, poor libido, increased reaction time and poor thrust etc. A significant (p<0.05) decrease in semen volume, sperm concentration, initial motility and number of semen doses was observed during first fortnight after development of lameness. No significant difference was observed in post thaw motility of ejaculate selected for freezing during 3 months study period. Percentage of thick ejaculates decreased while medium and thin ejaculates increased during first fortnight under the effect of lameness as compared to pre-lameness observations.
